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Hooke Kit™ MOG35-55/CFA Emulsion PTX

For EAE induction by active immunization in C57BL/6 mice

Product Selection | Protocol | Storage & Stability | Typical Results | Kit Contents | MSDS

Supplied in pre-filled syringes, ready to use.Product photo

EAE will develop in 90-100% of mice within 8 to 14 days, with mean maximum score of 3.0 to 3.5. Each batch is extensively tested and individually adjusted to ensure consistent disease induction.

On Day 0, MOG35-55/CFA emulsion and pertussis toxin are injected.
On Day 1, a second dose of pertussis toxin is injected.

Properly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.

Batch-to-batch reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.

Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.

Cat # Hooke Kit™ Strain PTX dose Description Kit Price
EK-0113 MOG35-55/CFA Emulsion PTX C57BL/6 1x Emulsion, pertussis toxin
in pre-filled syringes		 10 mice $ 220 ORDER
EK-0110 MOG35-55/CFA Emulsion PTX C57BL/6 1.5x Emulsion, pertussis toxin
in pre-filled syringes		 10 mice $ 220 ORDER
EK-0112 MOG35-55/CFA Emulsion PTX C57BL/6 2.5x Emulsion, pertussis toxin
in pre-filled syringes		 10 mice $ 240 ORDER
EK-0114 MOG35-55/CFA Emulsion PTX C57BL/6 3.75x Emulsion, pertussis toxin
in pre-filled syringes		 10 mice $ 260 ORDER
EK-0115 MOG35-55/CFA Emulsion PTX C57BL/6 5x Emulsion, pertussis toxin
in pre-filled syringes		 10 mice $ 260 ORDER

Product Selection (IMPORTANT)

Many factors – including mouse age, strain, breeder, and colony – influence EAE disease susceptibility and the pertussis toxin (PTX) dose required. Even with identical mice and reagents, EAE disease can vary considerably from lab to lab. 

Therefore, before ordering for the first time, we recommend that you run 10 mice with our strongest Hooke Kit™ (EK-0115) and let us recommend the best Hooke Kit™ for you based on the results (EAE incidence and average severity). Please contact us for information about sample ordering for this purpose.

Even using optimized reagents, our testing shows that Taconic mice develop somewhat more consistent EAE than Jackson mice. Mice of Taconic origin will have a more uniform time of onset and maximum severity score.

With a given Hooke Kit™, older mice generally develop more severe disease and more uniform disease onset.

Protocol

EAE Induction by Active Immunization in C57BL/6 Mice

Storage & Stability

Stable at least 1 month when stored at 2–4 °C

Typical Results

Results graph

EAE induction in C57BL/6 mice

Protocol: EAE Induction by Active Immunization in C57BL/6 Mice

Data are from three independent experiments using Hooke Kit™ MOG35-55/CFA Emulsion PTX (EK-0110), with Taconic mice.

Similar results are obtained using other MOG35-55/CFA Emulsion PTX Hooke Kits™ with the recommended mice.

Exp # Mice/group Age at
immunization		 Mean maximum
score ± SD		 Day of onset
± SD		 Disease
incidence
1 10 10 weeks 3.25 ± 0.49 10.9 ± 3.9 100 %
2 10 10 weeks 3.45 ± 0.60 10.1 ± 1.5 100 %
3 8 9 weeks 3.25 ± 0.38 12.1 ± 1.6 100 %

Detailed Kit Contents

Qty Description
2 1 ml syringes, pre-filled with MOG35-55/CFA emulsion
~ 1 mg MOG35-55/ml emulsion
~ 2 mg killed mycobacterium tuberculosis H37Ra/ml emulsion
2 1 ml syringes, pre-filled with pertussis toxin in phosphate buffered saline (PBS)
~ 1–6 µg pertussis toxin/ml of PBS
1 Data sheet: Recommended experimental protocol, typical results

Material Safety Data Sheets (MSDS)

MOG35-55/CFA Emulsion (PDF)

Pertussis Toxin in PBS (PDF)