Hooke Kits™ for Induction of EAE
For induction of experimental autoimmune encephalomyelitis (EAE) in mice
All reagents are supplied in pre-filled syringes, ready to use.
- Ensure reliable and consistent EAE induction
Each batch is extensively tested and individually adjusted to ensure consistent EAE induction.
- Eliminate tedious preparation of emulsions
Properly prepared emulsions are critical for reliable induction of EAE. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.
- Save time and mice in testing individual reagents
Batch-to-batch reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.
- Reduce your mouse facility’s exposure to pathogen contamination
Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.
Experimental autoimmune encephalomyelitis (EAE) is the model most commonly used to study efficacy of potential drugs for treatment of multiple sclerosis (MS).
Because of its many similarities to MS, EAE is used to study pathogenesis of autoimmunity, CNS inflammation, demyelination, cell trafficking and tolerance induction.
EAE is characterized by paralysis (in some models the paralysis is remitting-relapsing), CNS inflammation and demyelination. EAE is mediated by myelin-specific CD4+ T cells, but CD8+ cells and B cells may also play a role in some models of EAE.
We offer three antigens for induction of EAE:
- MOG35-55 – recommended for study of onset and development of EAE
- MOG1-125 – recommended for testing therapeutics which target B-cells
- PLP139-151 – recommended for study of EAE relapses
For information on model and antigen selection, please see our Learning Center.
MOG35-55/CFA Emulsion PTX for EAE induction in C57BL/6 mice
Product Selection | Protocol | Storage & Stability | Typical Results | Kit Contents | MSDS
Recommended for study of onset and development of EAE.
Larger doses of pertussis toxin (PTX) will induce more severe EAE. See product details for guidance on PTX dosage.
| Cat # | Hooke Kit™ | Strain | PTX dose | Description | Size | Price | |
|---|---|---|---|---|---|---|---|
| EK-0113 | MOG35-55/CFA Emulsion PTX | C57BL/6 | 1x | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 230 |  |
| EK-0110 | MOG35-55/CFA Emulsion PTX | C57BL/6 | 1.5x | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 230 | |
| EK-0112 | MOG35-55/CFA Emulsion PTX | C57BL/6 | 2.5x | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 250 | |
| EK-0114 | MOG35-55/CFA Emulsion PTX | C57BL/6 | 3.75x | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 270 | |
| EK-0115 | MOG35-55/CFA Emulsion PTX | C57BL/6 | 5x | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 280 | |
MOG1-125/CFA Emulsion PTX for EAE induction in C57BL/6 mice (NEW)
Product Selection | Protocol | Storage & Stability | Typical Results | Kit Contents | MSDS
Recommended for testing therapeutics which target B-cells.
Larger doses of pertussis toxin (PTX) will induce more severe EAE. See product details for guidance on PTX dosage.
| Cat # | Hooke Kit™ | Strain | PTX dose | Description | Size | Price | |
|---|---|---|---|---|---|---|---|
| EK-0162 | MOG1-125/CFA Emulsion PTX | C57BL/6 | 2.5x | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 1500 | |
| EK-0164 | MOG1-125/CFA Emulsion PTX | C57BL/6 | 3.75x | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 1520 | |
PLP139-151/CFA Emulsion (PTX) for EAE induction in SJL mice
Recommended for study of EAE relapses.
Induction with pertussis toxin (PTX) leads to higher incidence and severity of EAE in the first wave, but lower severity of relapse compared to induction without PTX.
EK-0120 or EK-0121 is recommended for studies primarily focusing on the relapse phase of EAE. EK-0123 is more suitable for evaluation of the initial wave of EAE. For more detailed information, see the recommended protocol.
| Cat # | Hooke Kit™ | Strain | Age | Description | Size | Price | |
|---|---|---|---|---|---|---|---|
| EK-0120 | PLP139-151/CFA Emulsion | SJL | 8–10 weeks | Emulsion in pre-filled syringes | 10 mice | $ 290 |
|
| EK-0121 | PLP139-151/CFA Emulsion PTX | SJL | 8–10 weeks | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 310 |
|
| EK-0123 | PLP139-151/CFA Emulsion PTXx2 | SJL | 8–10 weeks | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 330 |
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