Hooke Kit™ MOG35-55/CFA Emulsion PTX
For EAE induction by active immunization in C57BL/6 mice
Protocol | Storage & Stability | Typical Results | Kit Contents | MSDS
Supplied in pre-filled syringes, ready to use._240px.jpg)
- Consistently induces EAE in female C57BL/6 mice
EAE will develop in 90-100% of mice within 8 to 14 days, with mean maximum score of 3.0 to 3.5. Each batch is extensively tested and individually adjusted to ensure consistent disease induction.
On Day 0, MOG35-55/CFA emulsion and pertussis
toxin are injected.
On Day 1, a second dose of pertussis toxin is injected.
- Eliminates tedious preparation of emulsions
Properly prepared emulsions are critical for reliable induction of many autoimmune disease models. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.
- Saves time and mice in testing individual reagents
Batch-to-batch reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.
- Reduces your mouse facility’s exposure to pathogen contamination
Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.
| Cat # | Hooke Kit™ | Strain | Age | Description | Kit | Price | |
|---|---|---|---|---|---|---|---|
| EK-0110 | MOG35-55/CFA Emulsion PTX | C57BL/6 (Taconic) |
8–12 weeks | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 220 |  |
| EK-0112 | MOG35-55/CFA Emulsion PTX | C57BL/6 (JAX) |
10–14 weeks | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 240 | |
| EK-0113 | MOG35-55/CFA Emulsion PTX | C57BL/6 (Taconic) |
16–20 weeks | Emulsion, pertussis toxin in pre-filled syringes |
10 mice | $ 220 | |
Product Selection
Disease susceptibility varies according to mouse age and substrain; therefore we offer product variants optimized to match your mice.
Even using optimized reagents, our testing shows that Taconic mice develop somewhat more consistent EAE than Jackson mice. Mice of Taconic origin will have a more uniform time of onset and maximum severity score.
With a given Hooke Kit™, older mice generally develop more severe disease and more uniform disease onset.
Protocol
EAE Induction by Active Immunization in C57BL/6 Mice
Storage & Stability
Stable at least 1 month when stored at 2–4 °C
Typical Results
EAE induction in C57BL/6 mice
Protocol: EAE Induction by Active Immunization in C57BL/6 Mice
Data are from three independent experiments using Hooke Kit™ MOG35-55/CFA Emulsion PTX (EK-0110), with Taconic mice.
Similar results are obtained using EK-0112 and EK-0113 with the recommened mice.
| Exp # | Mice/group | Age at immunization |
Mean maximum score ± SD |
Day of onset ± SD |
Disease incidence |
|---|---|---|---|---|---|
| 1 | 10 | 10 weeks | 3.25 ± 0.49 | 10.9 ± 3.9 | 100 % |
| 2 | 10 | 10 weeks | 3.45 ± 0.60 | 10.1 ± 1.5 | 100 % |
| 3 | 8 | 9 weeks | 3.25 ± 0.38 | 12.1 ± 1.6 | 100 % |
Detailed Kit Contents
| Qty | Description |
|---|---|
| 2 | 1 ml syringes, pre-filled with MOG35-55/CFA emulsion
~ 1 mg MOG35-55/ml emulsion ~ 2 mg killed mycobacterium tuberculosis H37Ra/ml emulsion |
| 2 | 1 ml syringes, pre-filled with pertussis toxin in PBS 2 ± 1 µg pertussis toxin /ml of phosphate buffer saline (PBS) |
| 1 | Data sheet: Recommended experimental protocol, typical results |
Material Safety Data Sheets (MSDS)
MOG35-55/CFA Emulsion (PDF)
Pertussis Toxin in PBS (PDF)
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