Hooke Kits™ for Immunization

For induction of experimental autoimmune encephalomyelitis (EAE) in mice

All reagents are supplied in pre-filled syringes, ready to use.

Consistently induces strong immune response

Immune response is usually evaluated 10 to 14 days after immunization. Each batch is extensively tested and individually adjusted to ensure consistent immunization.

Eliminate tedious preparation of emulsions

Properly prepared emulsions are critical for reliable immunization. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.

Reduce your mouse facility’s exposure to pathogen contamination

Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.

Mice are immunized to induce antibody production, to test immune responses, or to expand antigen-specific T-cells for for adoptive transfer EAE. Immune response is usually evaluated 10 to 14 days after immunization. (See the recommended protocol for each product.)

Immunization using emulsions containing antigen in complete Freund’s adjuvant (CFA) results in a strong immune response against the antigen. A weak immune response is generated by emulsions containing antigen in incomplete Freund’s adjuvant (IFA).

Mice immunized with antigen in CFA will develop high antibody titer and a strong cellular immune response characterized by production of a wide variety of cytokines, of which Th1 are most dominant.

For antibody production, mice should be given a booster dose of antigen emulsified in IFA 2 to 4 weeks after initial immunization with antigen in CFA.