Antigens in TC media
For in vitro restimulation of T cells
- Tissue culture tested
- In tissue culture media, ready to use
Supplied in vials
- For analysis of T cell functions in vitro
Consistently induces in vitro recall responses of T cells isolated from immunized mice
- For adoptive transfer EAE
Induces potent encephalitogenic T cells in vitro
Antigens in TC media are used in vitro to generate encephalitogenic T-cells or to evaluate cellular immune responses.
Encephalitogenic cells are generated by culturing splenocytes from immunized mice for 72 hours in the presence of 10 to 20 µg/mL of antigen. To generate encephalitogenic cells from immunized C57B/6 mice, IL-12 and anti-IFNγ antibodies are added to the culture.
To evaluate cellular immune responses, either a cell suspension or purified CD4+ cells from draining lymph nodes or spleens are used. If purified CD4+ cells are used syngeneic antigen presenting cells (APC) are added. (APC are usually irradiated or mitomycin-C treated splenocytes from non-immunized mice.)
The cells are usually cultured in 96 well plates at 0.8–1.0x106 cells/well (4–5x106 cells/mL) with various concentrations of antigen (0.1 to 20 µg/mL). Proliferation of T-cells is usually evaluated after 48 hours of culture by adding and measuring [3H]thymidine incorporation. Cytokine production is evaluated in tissue culture supernatant after 48 to 72 hours of culture.
Antigens in TC Media
| Cat # | Product | Description | Size | MSDS (pdf) | Price |
|---|---|---|---|---|---|
| DS-0111 | MOG35-55 in TC Media, 100x | Peptide in tissue culture media | 2 mg in 1 mL | MSDS | $ 129 |
| DS-0121 | PLP139-151 in TC Media, 100x | Peptide in tissue culture media | 2 mg in 1 mL | MSDS | $ 119 |
| DS-0131 | MBP1-17 in TC Media, 100x | Peptide in tissue culture media | 2 mg in 1 mL | MSDS | $ 119 |
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