Fresh cells from immunized mice
For in vitro analysis of T cells, encephalitogenic cells, or culture for adoptive transfer EAE
Fresh splenocytes and/or lymph node cells from mice immunized with antigen.
- Perform in vitro primary cell assays without a vivarium
Fresh cell suspensions shipped cold overnight, ready for use.
- Improve robustness of in vitro assays
Consistent immunization and cell isolation protocols yield consistent cell quality.
- Save time establishing protocols, immunizing mice, isolating cells
Cells from CFA-only immunized mice and naïve mice available for comparison.
Lead time (IMPORTANT)
Orders must be placed 3 weeks before shipment. Each cell product order is custom prepared.
Preparation
Female mice of age 10 to 14 weeks are acclimated to our facility for at least 7 days, then immunized with antigen subcutaneously in the lower back, close to the base of tail, as follows:
| Strain | Hooke Kit™ used for antigen immunization |
Hooke Kit™ used for CFA-only immunization |
Hooke Kit™ used for naïve mice |
|---|---|---|---|
| C57BL/6 | EK-0111 (MOG35-55) | CK-0111 (CFA emulsion) | none |
| SJL | EK-0122 (PLP139-151) | CK-0122 (CFA emulsion) | none |
11 days later (10 days for SJL mice), mice are euthanized and cell suspension(s) from spleen and/or inguinal lymph nodes are prepared.
Red blood cells in spleen are lysed.
Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.
Cells are shipped the same day for overnight delivery.
Cells from antigen immunized mice
| Cat # | Strain | Antigen | Product | Size | Price (first mouse) |
Price (each add'l mouse) |
|---|---|---|---|---|---|---|
| CF-1001 | C57BL/6 | MOG35-55 | Spleen cells | 1 mouse | $ 125 | $ 82 |
| CF-1002 | C57BL/6 | MOG35-55 | Lymph node cells | 1 mouse | $ 125 | $ 82 |
| CF-1003 | C57BL/6 | MOG35-55 | Spleen cells and lymph node cells | 1 mouse | $ 165 | $ 108 |
| CF-2001 | SJL | PLP139-151 | Spleen cells | 1 mouse | $ 139 | $ 91 |
| CF-2002 | SJL | PLP139-151 | Lymph node cells | 1 mouse | $ 139 | $ 91 |
| CF-2003 | SJL | PLP139-151 | Spleen cells and lymph node cells | 1 mouse | $ 179 | $ 117 |
Cells from CFA-only immunized (control) mice
| Cat # | Strain | Antigen | Product | Size | Price (first mouse) |
Price (each add'l mouse) |
|---|---|---|---|---|---|---|
| CF-1101 | C57BL/6 | CFA only | Spleen cells | 1 mouse | $ 125 | $ 82 |
| CF-1102 | C57BL/6 | CFA only | Lymph node cells | 1 mouse | $ 125 | $ 82 |
| CF-1103 | C57BL/6 | CFA only | Spleen cells and lymph node cells | 1 mouse | $ 165 | $ 108 |
| CF-2101 | SJL | CFA only | Spleen cells | 1 mouse | $ 139 | $ 91 |
| CF-2102 | SJL | CFA only | Lymph node cells | 1 mouse | $ 139 | $ 91 |
| CF-2103 | SJL | CFA only | Spleen cells and lymph node cells | 1 mouse | $ 179 | $ 117 |
Cells from non-immunized (naïve) mice
| Cat # | Strain | Antigen | Product | Size | Price (first mouse) |
Price (each add'l mouse) |
|---|---|---|---|---|---|---|
| CF-1201 | C57BL/6 | none | Spleen cells | 1 mouse | $ 100 | $ 65 |
| CF-1202 | C57BL/6 | none | Lymph node cells | 1 mouse | $ 100 | $ 65 |
| CF-1203 | C57BL/6 | none | Spleen cells and lymph node cells | 1 mouse | $ 140 | $ 91 |
| CF-2201 | SJL | none | Spleen cells | 1 mouse | $ 114 | $ 75 |
| CF-2202 | SJL | none | Lymph node cells | 1 mouse | $ 114 | $ 75 |
| CF-2203 | SJL | none | Spleen cells and lymph node cells | 1 mouse | $ 154 | $ 101 |
Controls
For some assays (i.e. protein and gene expression analysis) spleen or lymph node cells from naïve mice or mice immunized with CFA-only should be used as negative controls.
Detailed contents
Centrifuge tubes (15 or 50 mL) with cells in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10-5 M 2-mercaptoethanol).
Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cells will be pooled in one tube with one cell count.
(Please specify if you want separate tubes for each mouse instead of pooled cells; in that case the "first mouse" price applies.)
Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.
| Cell type | Cells/mouse (immunized) |
Cells/mouse (naïve) |
|---|---|---|
| Splenocytes | 100-200 x 106 | 50-100 x 106 |
| Lymph node cells | 10-30 x 106 | 1-3 x 106 |
Storage & stability
Fresh cells ship cold on gelpaks in insulated packaging. They should be used or frozen within 24 hours of shipment.
For this reason this product ships to US and Canadian addresses only, via FedEx overnight.
(We can supply frozen cells to international customers at an additional charge of $25/item. These ship on dry ice. Please specify "frozen cells" when ordering.)
Material Safety Data Sheet (MSDS)
Related Products
DS-0111 MOG35-55 in TC Media, 100x
DS-0121 PLP139-151 in TC Media, 100x
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